3QRP
Structure of Thermus Thermophilus Cse3 bound to an RNA representing a product mimic complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.057, 72.405, 100.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.752 - 2.352 |
| R-factor | 0.1774 |
| Rwork | 0.175 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.518 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.752 | 2.430 |
| High resolution limit [Å] | 2.352 | 2.352 |
| Number of reflections | 11249 | |
| Completeness [%] | 95.5 | 75.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 15% PEG 8000, 100mM KCl, 5mM MgSO4, 1mM spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
