3QR9
Anthranilate phosphoribosyltransferase (trpD) from Mycobacterium tuberculosis (apo structure)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.465, 91.771, 120.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.870 - 1.870 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.21449 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.460 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.870 | 26.488 | 1.960 |
| High resolution limit [Å] | 1.860 | 5.890 | 1.860 |
| Rmerge | 0.023 | 0.741 | |
| Number of reflections | 72847 | ||
| <I/σ(I)> | 19.4 | 23.2 | 1 |
| Completeness [%] | 98.1 | 98.8 | 87.6 |
| Redundancy | 6.9 | 6.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2M imidazole malate, 15% PEG4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
