3QR8
Crystal structure of the bacteriophage P2 membrane-piercing protein gpV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-09-09 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 68.539, 68.539, 132.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.090 - 2.030 |
| R-factor | 0.2619 |
| Rwork | 0.260 |
| R-free | 0.30500 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.996 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (C/D/E) |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.090 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.069 | 0.362 |
| Number of reflections | 23833 | |
| <I/σ(I)> | 27.6 | 7.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 17.4 | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 30 mg/mL protein, 0.3-0.6 M lithium sulfate, 4-6% PEG8000, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
