3QR1
Crystal Structure of L. pealei PLC21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-08-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.367, 148.869, 151.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 3.200 |
| R-factor | 0.21608 |
| Rwork | 0.214 |
| R-free | 0.25776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.299 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.360 | 3.100 |
| Rmerge | 0.146 | 0.039 | 0.814 |
| Number of reflections | 34741 | ||
| <I/σ(I)> | 5.4 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 7.2 | 6.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | 100mM Bis-Tris, 300 mM NaCl, 35% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
