3QQI
Crystal structure of the HA1 receptor binding domain of H2 hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.629, 62.318, 66.034 |
| Unit cell angles | 64.25, 80.04, 72.12 |
Refinement procedure
| Resolution | 36.720 - 1.650 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.120 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.053 | 0.037 | 0.244 |
| Number of reflections | 60290 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 94.8 | 96.9 | 83.8 |
| Redundancy | 1.9 | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 295.5 | 1.25 Sodium Citrate, 0.1M HEPES, pH 8.1, vapor diffusion, sitting drop, temperature 295.5K |
