3QQE
Crystal structure of HA2 R106H mutant of H2 hemagglutinin, re-neutralized form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-21 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97915 |
Spacegroup name | P 63 |
Unit cell lengths | 70.339, 70.339, 237.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.000 - 2.100 |
R-factor | 0.1913 |
Rwork | 0.189 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.337 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.3.3) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.084 | 0.050 | 0.514 |
Number of reflections | 37509 | ||
<I/σ(I)> | 15 | ||
Completeness [%] | 97.6 | 99.7 | 81.8 |
Redundancy | 7.8 | 11 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 295.5 | 24% PEG 3000, 0.1M Tris, pH 9.0, vapor diffusion, sitting drop, temperature 295.5K |