3QQ8

Crystal structure of p97-N in complex with FAF1-UBX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 65
Unit cell lengths	88.080, 88.080, 66.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.135 - 2.000
R-factor	0.1686
Rwork	0.166
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1S3S 1h8c
RMSD bond length	0.007
RMSD bond angle	1.103
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	PHASER
Refinement software	PHENIX (1.6.2_432)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.279	38.140	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.077	0.036	0.534
Number of reflections	19757
<I/σ(I)>	16.5	14.6	1.4
Completeness [%]	98.5	99.4	97.8
Redundancy	7.7	7.3	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	16-20% PEG MME 2000, 0.2 M Trimethyl-N-oxide, 0.1 M Tris, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.5