Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.99 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 560.120, 560.120, 560.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 5.200 |
| R-factor | 0.461 |
| Rwork | 0.461 |
| R-free | 0.46700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | RAVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 5.290 |
| High resolution limit [Å] | 5.200 | 5.200 |
| Rmerge | 0.080 | 0.261 |
| Number of reflections | 86130 | |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 77.5 | 3.5 |
| Redundancy | 2.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 4.0% PEG 4000, 0.2M sodium acetate pH 4.6, 0.3M calcium chloride, 15% glycerol, 0.1M sarcosine, 6% n-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
