3QPQ

Crystal structure of ANTI-TLR3 antibody C1068 FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-09
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	82.480, 136.940, 83.250
Unit cell angles	90.00, 114.95, 90.00

Refinement procedure

Resolution	44.553 - 1.900
R-factor	0.2041
Rwork	0.201
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.111
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNX
Refinement software	PHENIX ((phenix.refine: dev_601))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Rmerge	0.120
Number of reflections	119205
Completeness [%]	90.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1 M Tris, pH 8.5, 22% PEG 8000, 0.2 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K