3QPL
G106W mutant of EthR from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.740, 119.740, 33.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 3.200 |
| R-factor | 0.24378 |
| Rwork | 0.240 |
| R-free | 0.31768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.408 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.000 | 10.000 | 3.500 |
| High resolution limit [Å] | 3.180 | 6.000 | 3.180 |
| Number of reflections | 29164 | ||
| Completeness [%] | 8.9 | 84.4 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | glycerol, polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
