3QPA
Structure of Fusarium Solani Cutinase expressed in Pichia pastoris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.77009 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.006, 66.165, 36.755 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 13.690 - 0.850 |
| R-factor | 0.1101 |
| Rwork | 0.110 |
| R-free | 0.11690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cex |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.499 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.690 | 0.860 |
| High resolution limit [Å] | 0.850 | 0.850 |
| Number of reflections | 141550 | |
| Completeness [%] | 96.4 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% (w/v) PEG6000, 0.1M citric acid, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
