3QP8
Crystal structure of CviR (Chromobacterium violaceum 12472) ligand-binding domain bound to C10-HSL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.461, 71.613, 77.737 |
| Unit cell angles | 90.00, 114.58, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.600 |
| R-factor | 0.1991 |
| Rwork | 0.198 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qp6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.710 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.397 | |
| Number of reflections | 101162 | |
| <I/σ(I)> | 16.678 | 2.25 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 296 | 100 mM CHES, 1 M sodium citrate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
