3QP6
Crystal structure of CviR (Chromobacterium violaceum 12472) bound to C6-HSL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.248, 89.120, 132.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.536 - 2.000 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QP7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.061 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.319 | |
| Number of reflections | 20522 | |
| <I/σ(I)> | 17.127 | 4.656 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 100 mM imidazole, 10% w/v PEG5000 MME, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
