3QOY

Crystal structure of ribosomal protein L1 from Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2011-01-19
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54189
Spacegroup name	C 1 2 1
Unit cell lengths	107.490, 37.520, 58.970
Unit cell angles	90.00, 110.32, 90.00

Refinement procedure

Resolution	19.653 - 2.100
R-factor	0.1967
Rwork	0.194
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ad2
RMSD bond length	0.008
RMSD bond angle	1.146
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.670	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.116	0.332
Number of reflections	12221
<I/σ(I)>	11.94	2.85
Completeness [%]	92.9	83.3
Redundancy	3.39	1.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	285	25 % PEG4000, 100mM acetate buffer, 200mM ammonium sulphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 285K