3QOU
Crystal Structure of E. coli YbbN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.523, 62.447, 79.981 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 79.460 - 1.800 |
| R-factor | 0.1894 |
| Rwork | 0.187 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r5s |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.013 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.051 | 0.028 | 0.367 |
| Number of reflections | 25553 | ||
| <I/σ(I)> | 11.4 | 2.3 | |
| Completeness [%] | 95.0 | 97.1 | 79.2 |
| Redundancy | 3.6 | 3.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% PEG8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
