3QOO
Crystal structure of hot-dog-like Taci_0573 protein from Thermanaerovibrio acidaminovorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791829 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.078, 38.078, 142.148 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.380 - 1.250 |
| R-factor | 0.1567 |
| Rwork | 0.156 |
| R-free | 0.18051 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.476 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.138 | 0.582 |
| Number of reflections | 34179 | |
| <I/σ(I)> | 23.6 | 2.1 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 10.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 M MgCl2, 0.1 M sodium citrate, 40% PEG400, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
