3QOM
Crystal structure of 6-phospho-beta-glucosidase from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791829 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 150.647, 150.647, 95.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.048 - 1.498 |
| R-factor | 0.1184 |
| Rwork | 0.118 |
| R-free | 0.13410 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.455 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: dev_601)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.112 | 0.650 |
| Number of reflections | 102382 | |
| <I/σ(I)> | 33.2 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.7 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 297 | 0.1 M sodium acetate, 0.8 M NaH2PO4/1.2 M K2HPO4, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
