3QOJ
Cryogenic structure of Staphylococcal nuclease variant D+PHS/V23K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.138, 60.351, 38.156 |
| Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
| Resolution | 23.650 - 1.600 |
| R-factor | 0.18888 |
| Rwork | 0.187 |
| R-free | 0.22762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.650 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | |
| Number of reflections | 18219 | |
| <I/σ(I)> | 22.4 | 6.3 |
| Completeness [%] | 97.5 | 97 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 24% MPD, 25 mM potassium phosphate, pdTp (2 eq.), calcium chloride (3 eq.), pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
