3QO7

Crystal structure of the seryl-tRNA synthetase from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-07
Detector	ADSC QUANTUM 210
Spacegroup name	P 61 2 2
Unit cell lengths	91.152, 91.152, 275.839
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.973 - 2.550
R-factor	0.1795
Rwork	0.178
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dq0
RMSD bond length	0.011
RMSD bond angle	1.303
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.100	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.085	0.570
Number of reflections	23155
<I/σ(I)>	8	1.3
Completeness [%]	100.0	100
Redundancy	13.6	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	3.2-3.4 M ammonium sulfate, 0-2% v/v glycerol, 100 mM MES/sodium, pH 5.6-5.8, VAPOR DIFFUSION, temperature 298K