3QO5
Crystal Structure of the seryl-tRNA synthetase from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.461, 92.461, 273.566 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.103 - 2.300 |
| R-factor | 0.1913 |
| Rwork | 0.190 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dq0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.334 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.100 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.098 | 0.440 |
| Number of reflections | 31871 | |
| <I/σ(I)> | 6 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.6 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 3.2-3.4 M ammonium sulfate, 0-2% v/v glycerol, 100 mM MES/sodium, pH 5.6-5.8, VAPOR DIFFUSION, temperature 298K |
