3QO4

The Crystal Structure of Death Receptor 6

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-03
Detector	RAYONIX MX-225
Wavelength(s)	0.918, 1.03936, 1.04008, 1.04064
Spacegroup name	P 61 2 2
Unit cell lengths	77.910, 77.910, 187.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.700 - 2.200
Rwork	0.215
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.610
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.982	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.064	0.325
Number of reflections	20455
<I/σ(I)>	11.2	3
Completeness [%]	99.6	98.2
Redundancy	3.2	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M Sodium citrate, 30% PEG4000, 0.5 M Ammoniumacetate , pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K