3QO3
Crystal structure of Escherichia coli Hfq, in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.300, 40.620, 100.810 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.150 |
| R-factor | 0.2294 |
| Rwork | 0.228 |
| R-free | 0.25727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.670 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.111 | 0.432 |
| Number of reflections | 21783 | |
| <I/σ(I)> | 17.96 | 3.7 |
| Completeness [%] | 95.1 | 88.9 |
| Redundancy | 5.61 | 5.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M Hepes, 10% (W/V) PEG 8000, 8% (V/V) Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
