3QNQ
Crystal structure of the transporter ChbC, the IIC component from the N,N'-diacetylchitobiose-specific phosphotransferase system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08097 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 132.812, 132.812, 452.954 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.974 - 3.295 |
R-factor | 0.2294 |
Rwork | 0.228 |
R-free | 0.26360 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.217 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
High resolution limit [Å] | 3.295 | 7.100 | 3.300 |
Rmerge | 0.077 | 0.042 | 0.702 |
Number of reflections | 59563 | ||
<I/σ(I)> | 7.9 | ||
Completeness [%] | 95.7 | 99.5 | 91.4 |
Redundancy | 10.1 | 12.9 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30% PEG 400, 100 mM Li2SO4, 0.5% polyvinylpyrrolidone, 100 mM Na Citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
2 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30% PEG 400, 100 mM Li2SO4, 0.5% polyvinylpyrrolidone, 100 mM Na Citrate, Ta6Br12 powder., pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |