3QNN
RB69 DNA Polymerase (Y567A) Ternary Complex with dGT Opposite 3tCo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.811, 120.042, 130.409 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| R-factor | 0.18244 |
| Rwork | 0.181 |
| R-free | 0.21366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.134 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 85100 | |
| Completeness [%] | 94.5 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 150 mM CaCl2, 15%(w/v) PEG350 monomethyl ether (MME), and 100mM sodium cacodylate (pH6.5), VAPOR DIFFUSION, temperature 298K |
