3QN0
Structure of 6-pyruvoyltetrahydropterin synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 112.853, 117.680, 153.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.571 - 2.340 |
| R-factor | 0.2244 |
| Rwork | 0.222 |
| R-free | 0.26659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qn9 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.003 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.470 | |
| High resolution limit [Å] | 2.340 | 2.343 |
| Rmerge | 0.094 | 0.094 |
| Number of reflections | 43356 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.16M tri-ammonium citrate pH 7.0, 16 % PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
