3QMZ

Crystal structure of the cytoplasmic dynein heavy chain motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.21476 1.15776
Spacegroup name	P 1 21 1
Unit cell lengths	174.080, 118.921, 200.509
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	50.000 - 6.000
Rwork	0.430
R-free	0.43000
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.130
High resolution limit [Å]	6.000
Number of reflections	20819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	277	16% PEG3350, 0.1 M sodium citrate, 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.7
1	VAPOR DIFFUSION, HANGING DROP	5.7	277	16% PEG3350, 0.1 M sodium citrate, 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.7