3QMN
Crystal Structure of 4'-Phosphopantetheinyl Transferase AcpS from Vibrio cholerae O1 biovar eltor
Replaces: 3OTAReplaces: 3H88Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.553, 139.039, 138.319 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 29.890 - 1.850 |
| R-factor | 0.15791 |
| Rwork | 0.156 |
| R-free | 0.19127 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.755 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.550 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.540 | |
| Number of reflections | 300465 | |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 30% MPD, 0.1 M Sodium Acetate pH 4.6, 20 mM Calcium Chloride, 5 mM Coenzyme A, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
