3QMF
Crystal strucuture of an inositol monophosphatase family protein (SAS2203) from Staphylococcus aureus MSSA476
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.983, 68.350, 143.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.670 - 2.600 |
| R-factor | 0.1984 |
| Rwork | 0.195 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p3n |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.612 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 71.894 |
| High resolution limit [Å] | 2.597 |
| Number of reflections | 15691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2M Lithium sulphate, 0.1M HEPES pH 7.0, 20%(w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
