3QME
Crystal Structure of an Anopheles gambiae Odorant-binding Protein AgamOBP22a with Bound Odorant Cyclohexanone
Replaces: 3L5GExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-09 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.986, 37.593, 43.618 |
| Unit cell angles | 90.00, 99.17, 90.00 |
Refinement procedure
| Resolution | 25.487 - 2.002 |
| R-factor | 0.1733 |
| Rwork | 0.169 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l4a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.931 |
| Data reduction software | DENZO |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.420 | 2.000 |
| Rmerge | 0.069 | 0.038 | 0.285 |
| Number of reflections | 7597 | ||
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 96.1 | 98.1 | 93.6 |
| Redundancy | 7 | 6.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium citrate tribasic, MPD, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
