3QMD
Structural Basis of Selective Binding of Nonmethylated CpG Islands by the CXXC Domain of CFP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 30.653, 74.688, 125.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.110 - 1.900 |
| R-factor | 0.2254 |
| Rwork | 0.225 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 3QMB |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.662 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.067 | 0.044 | 0.512 |
| Number of reflections | 11621 | ||
| <I/σ(I)> | 12.7 | ||
| Completeness [%] | 97.8 | 99.8 | 88 |
| Redundancy | 5.5 | 5.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M Hepes pH 7.5, 0.1M MgCl2, 30% PEG550MME., VAPOR DIFFUSION, HANGING DROP, temperature 291K |
