3QM9
Blackfin tuna azido-myoglobin, atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.008, 51.894, 38.644 |
| Unit cell angles | 90.00, 103.95, 90.00 |
Refinement procedure
| Resolution | 17.270 - 0.910 |
| R-factor | 0.12068 |
| Rwork | 0.120 |
| R-free | 0.13324 |
| Structure solution method | RIGID BODY refinement |
| Starting model (for MR) | 2nrl |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.270 | 0.934 |
| High resolution limit [Å] | 0.910 | 0.910 |
| Rmerge | 0.056 | 0.301 |
| Number of reflections | 82720 | |
| <I/σ(I)> | 31.21 | 5.9 |
| Completeness [%] | 93.7 | 87.34 |
| Redundancy | 5.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M potassium/sodium tartrate, 0.1 M sodium citrate, 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
