3QM3
1.85 Angstrom Resolution Crystal Structure of Fructose-bisphosphate Aldolase (Fba) from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.996, 101.971, 105.662 |
| Unit cell angles | 113.27, 95.34, 95.75 |
Refinement procedure
| Resolution | 29.660 - 1.850 |
| R-factor | 0.15097 |
| Rwork | 0.149 |
| R-free | 0.19088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dos |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.092 | 0.571 |
| Number of reflections | 242214 | |
| <I/σ(I)> | 21 | 3.7 |
| Completeness [%] | 97.7 | 96.4 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Protein solution: 0.3M Sodium chloride, 10mM HEPES (pH 7.5) Screen solution: 0.2M NH4 Sulfate, 0.1M Na Cacodylate pH 7.5, 30%PEG 2K MME, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
