3QM2

2.25 Angstrom Crystal Structure of Phosphoserine Aminotransferase (SerC) from Salmonella enterica subsp. enterica serovar Typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	64.260, 82.171, 71.925
Unit cell angles	90.00, 95.83, 90.00

Refinement procedure

Resolution	29.950 - 2.250
R-factor	0.18833
Rwork	0.185
R-free	0.24235
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bjn
RMSD bond length	0.013
RMSD bond angle	1.452
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.077	0.477
Number of reflections	35281
<I/σ(I)>	14.5	2.3
Completeness [%]	99.9	99.2
Redundancy	3.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Protein: 7.5mGr/mL, 0.5M Sodium chloride, 0.01M TRIS-HCL pH 8.3, Screen: PACT (G1), 0.2M Sodium fluoride, 0.1M Bis Tris propane pH 7.5, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K