3QLV
Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD tetramer assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 366.552, 109.176, 155.469 |
| Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
| Resolution | 48.950 - 3.940 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3OM0 AND 3H6H |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.100 |
| High resolution limit [Å] | 3.940 | 3.950 |
| Rmerge | 0.139 | 0.895 |
| Number of reflections | 54061 | |
| <I/σ(I)> | 18.6 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 10% Ethylene Glycol, 0.1 M HEPES, 5% PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
