3QLI
Crystal Structure of RipA from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-11-14 |
| Detector | ADSC Q315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.586, 108.307, 84.970 |
| Unit cell angles | 90.00, 120.10, 90.00 |
Refinement procedure
| Resolution | 41.230 - 1.900 |
| R-factor | 0.1725 |
| Rwork | 0.172 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.042 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.053 | 0.048 | 0.159 |
| Number of reflections | 71782 | ||
| <I/σ(I)> | 19.2 | ||
| Completeness [%] | 98.1 | 96.4 | 86.2 |
| Redundancy | 3.3 | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 8% tacsimate pH 5.0, 8% PEG 3350, 4% v/v 2,5-hexandiol and 1mM Succinyl coenzyme A , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
