3QKS

Mre11 Rad50 binding domain bound to Rad50

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-01
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	55.779, 91.232, 107.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.670 - 2.100
R-factor	0.2125
Rwork	0.210
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ii8
RMSD bond length	0.003
RMSD bond angle	0.679
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.670	2.168
High resolution limit [Å]	2.100	2.100
Rmerge	0.051	0.457
Number of reflections	31661
<I/σ(I)>	27.5	2.8
Completeness [%]	96.8	86
Redundancy	4.6	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	100 mM Tris, 200 mM magnesium chloride, 20% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K