3QJB
Human Hemoglobin A Mutant Alpha H58L Carbonmonoxy-Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-30 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.600, 53.600, 191.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.178 - 1.800 |
| R-factor | 0.1729 |
| Rwork | 0.170 |
| R-free | 0.20480 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2dn3 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.989 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.000 | 99.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Number of reflections | 26524 | ||
| <I/σ(I)> | 32.1 | 12.5 | |
| Completeness [%] | 98.1 | 96.8 | 96.9 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 293 | 2.3 M Na/K phosphate, pH 6.7, SMALL TUBES, temperature 293K |
