3QJ6

The crystal structure of PWWP domain of human Hepatoma-derived growth factor 2 in complex with H3K79me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97944
Spacegroup name	P 61
Unit cell lengths	74.934, 74.934, 41.764
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.468 - 2.300
R-factor	0.236
Rwork	0.234
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eae
RMSD bond length	0.011
RMSD bond angle	1.173
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.085	0.053	0.466
Number of reflections	6094
<I/σ(I)>	11.6
Completeness [%]	100.0	99.7	100
Redundancy	10.8	9.9	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	2.0M ammonium sulfate, 5% isopropanol. Protein was pre-incubated with 5 equivalents of peptide ligand, VAPOR DIFFUSION, temperature 293K