3QIR
Crystal structure of PIWIL2 PAZ domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28286 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.548, 139.291, 224.934 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.750 - 2.450 |
| R-factor | 0.2428 |
| Rwork | 0.242 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.077 | 0.854 |
| Number of reflections | 24387 | |
| <I/σ(I)> | 38.6 | 1.8 |
| Completeness [%] | 98.4 | 93.1 |
| Redundancy | 7.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.2 M NaI, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
