3QII

Crystal structure of tudor domain 2 of human PHD finger protein 20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97911
Spacegroup name	P 43 21 2
Unit cell lengths	48.683, 48.683, 96.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.237
Rwork	0.235
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o4x
RMSD bond length	0.016
RMSD bond angle	1.361
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.069	0.030	0.318
Number of reflections	5606
<I/σ(I)>	12.8
Completeness [%]	99.2	99.1	86.8
Redundancy	12.3	11.2	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	25% PEG-8000, 0.2M sodium chloride, 0.1M TRIS, pH 8.5, vapor diffusion, sitting drop, temperature 291K