3QH9
Human Liprin-beta2 Coiled-Coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4938 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 29.964, 162.656, 34.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.380 - 2.010 |
| R-factor | 0.2348 |
| Rwork | 0.232 |
| R-free | 0.29780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.380 | 80.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.099 | 0.071 | 0.348 |
| Number of reflections | 5774 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 97.0 | 96.4 | 84.1 |
| Redundancy | 10.2 | 10 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 190 mM ammonium iodide, 23% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
