3QH3
The crystal structure of TCR A6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.247, 51.735, 96.336 |
| Unit cell angles | 90.00, 105.05, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.190 |
| R-factor | 0.20527 |
| Rwork | 0.202 |
| R-free | 0.27411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.240 |
| High resolution limit [Å] | 2.190 | 5.920 | 2.190 |
| Rmerge | 0.070 | 0.032 | 0.578 |
| Number of reflections | 44707 | ||
| <I/σ(I)> | 16.6 | ||
| Completeness [%] | 99.6 | 99.5 | 99.1 |
| Redundancy | 3.5 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | PEG 30%, TRIS 0.1M, pH 8.5, vapor diffusion, sitting drop, temperature 297K |
