3QGU
L,L-Diaminopimelate aminotransferase from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.952, 91.800, 162.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.12558 |
| Rwork | 0.125 |
| R-free | 0.17077 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z20 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.989 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 122383 | |
| Completeness [%] | 97.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 200mM lithium sulfate, 25%(w/v) polyethylene glycol 3350, 100mM Bis-Tris propane, 0.02%(w/v) sodium azide, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
