3QGM
p-nitrophenyl phosphatase from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.252, 68.843, 70.154 |
| Unit cell angles | 74.99, 87.33, 85.76 |
Refinement procedure
| Resolution | 37.200 - 2.000 |
| R-factor | 0.1715 |
| Rwork | 0.169 |
| R-free | 0.21340 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.578 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.200 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.090 | 0.074 | 0.522 |
| Number of reflections | 62618 | ||
| <I/σ(I)> | 11.1 | 1.99 | |
| Completeness [%] | 95.1 | 99 | 62.7 |
| Redundancy | 3.8 | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 294 | 0.1 M Tris buffer, 0.2 M calcium chloride, 26% PEG-3350, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
