3QGL
Crystal Structure of PDZ domain of sorting nexin 27 (SNX27) in complex with the ESESKV peptide corresponding to the C-terminal tail of GIRK3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.571, 81.567, 90.678 |
| Unit cell angles | 90.00, 102.11, 90.00 |
Refinement procedure
| Resolution | 38.949 - 3.310 |
| R-factor | 0.23462 |
| Rwork | 0.234 |
| R-free | 0.25434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qe1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.207 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.949 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.093 | 0.208 |
| Number of reflections | 9810 | |
| <I/σ(I)> | 14.72 | 6.08 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 4.0 M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
