3QGC

Crystal structure of FBF-2 R288Y mutant in complex with gld-1 FBEa A7U mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	96.577, 96.577, 101.389
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.600 - 1.900
Rwork	0.181
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5y
RMSD bond length	0.004
RMSD bond angle	0.649
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.600	1.930
High resolution limit [Å]	1.900	1.900
Rmerge		0.550
Number of reflections	42132
<I/σ(I)>	14.1	5.6
Completeness [%]	99.7	99.5
Redundancy	11.5	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 MM TRIS PH 7.5, 10% POLYETHYLENE GLYCOL 8000, AND 8% ETHYLENE GLYCOL, VAPOR DIFFUSION, HANGING DROP, temperature 298K