3QG9

crystal structure of FBF-2/gld-1 FBEa A7U mutant complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-25
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	96.750, 96.750, 101.175
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.450 - 2.250
Rwork	0.170
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.957
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.146	0.588
Number of reflections	25563
<I/σ(I)>	17.1	3.7
Completeness [%]	99.9	100
Redundancy	10.8	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	8% PEG8000, 8% ethylene glycol, 0.1 M Tris (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K