3QFY
Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.710, 98.254, 104.450 |
Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
Resolution | 38.320 - 2.300 |
R-factor | 0.14121 |
Rwork | 0.138 |
R-free | 0.20839 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.020 |
RMSD bond angle | 1.703 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 74187 | |
<I/σ(I)> | 13 | 3.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.6M ammonium sulfate, 0.1M HEPES-NaOH, 10mM glucose, 1mM isofagomine, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |