3QFW

Crystal structure of Rubisco-like protein from Rhodopseudomonas palustris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	57.440, 119.217, 66.856
Unit cell angles	90.00, 115.46, 90.00

Refinement procedure

Resolution	27.921 - 1.789
R-factor	0.2457
Rwork	0.244
R-free	0.28400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.042
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	27.921
High resolution limit [Å]	1.789
Number of reflections	71923
Completeness [%]	94.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG 3350, 0,1M Bis-Tris, 0.2M Lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K