3QFL

Coiled-Coil Domain-Dependent Homodimerization of Intracellular MLA Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	298
Detector technology	CCD
Collection date	2009-06-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9767
Spacegroup name	P 21 21 2
Unit cell lengths	97.462, 30.268, 38.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.707 - 1.997
R-factor	0.2122
Rwork	0.210
R-free	0.25840
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.924
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.997	2.000
Number of reflections	8809
Completeness [%]	99.4	99.6
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1M sodium acetate trihydrate(NaAc), 2.0M sodium formate(NaCOOH), pH 4.6, VAPOR DIFFUSION, HANGING DROP , temperature 298K